• Product Name: Varioskan Flash Multi functional Reader
• Order number: tlyon000393
• Brand Name: Thermo, USA
• Type: Multi functional ELISA reader
• Filter position: None
• Incubator: available
• Printer: External

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Varioskan Flash， The latest upgraded full wavelength scanning multifunctional reader has the widest spectrum of fluorescence, time-resolved fluorescence, colorimetric, and chemiluminescence reading functions, and can be configured with up to 3 built-in liquid separators. Its excellent optical performance and optimized overall software performance can help you achieve various applications and obtain ideal test results.

High performance optical system, achieving unparalleled high signal-to-noise ratio

The optical system optimized with Varioskan Flash enables unlimited wavelength step selection for any detection method, achieving maximum sensitivity and a wide dynamic measurement range.

In fluorescence reading, the four monochromator and quartz fiber system minimize stray light while also minimizing optical loss, improving signal-to-noise ratio and sensitivity. The optional top or bottom reading function provides more flexible selection of experimental conditions.

In colorimetric reading, the design of dual monochromators also minimizes stray light, ensuring excellent linearity and accuracy. Combined with its baseline noise suppression function, it ensures accurate results for measuring extremely low concentration samples.

When measuring chemiluminescence, a dark current quantum counting detector is used, with two standard measurement components: using a grating scanning mode to determine the optimal wavelength and optimize the measurement parameters; LumiSens high sensitivity module can provide the highest sensitivity, especially in multiple label readings.

Built in up to 3 liquid separators, capable of quickly adding liquid

For accurate and convenient allocation of reagents, up to 3 built-in dispensers can be optionally selected. The instrument supports synchronous liquid separation and measurement, and tracks the entire reaction process in real-time from the beginning of the kinetic reaction. This feature is particularly significant in flash reactions, Ca+channels, and other rapid kinetic applications.

Widely applicable, even UV fluorescence measurement can ensure results

Due to the broad wavelength range of Varioskan Flash, it is highly suitable for measurement experiments ranging from low UV ultraviolet to near-infrared. As a photometer with a low UV zone, Varioskan also has optical path calibration function, which can directly measure nucleic acid concentration and purity. In UV fluorescence assays such as measuring tryptophan or tyrosine fluorescence, which are used in fluorescence free labeling experiments, protein structural changes can be effectively detected.

Varioskan Flash has top/low read function, comes with high-precision incubator, can read various plate types, and can perform single hole multi-point scanning. Therefore, it is highly suitable for cell-based fluorescence assays.

High performance three position incubator for better control of detection conditions

The Varioskan Flash comes with a high-performance built-in incubator and a unique three position incubation system that prevents the formation of moisture beneath the porous plate cover, allowing for direct measurement of the covered porous plate.

Varioskan also comes with a ring rail oscillator that can adjust the oscillation speed and diameter arbitrarily, ensuring effective mixing and accelerating reaction speed.

Specially designed to be compatible with fully automated systems

The design of Varioskan Flash allows for easy integration with fully automated systems. The special design of its pallet plate allows the robotic arm to easily bend the board horizontally or vertically.

Skanlt software provides logical experimental program settings

The powerful Skanlt software simplifies the process of setting up the optimal experimental program, handles data flexibly, and provides different report formats. The unique step table function makes program settings both intuitive and flexible, and the process is very logical and easy to understand.

application

● Cytological testing

● Ca2+channel detection

● Report gene experiments

● Cell apoptosis

● Cell proliferation

● Cytotoxicity and ADMETO

● Signal transduction

● Intermolecular interactions

Tryptophan and tyrosine fluorescence

Europium labeling experiment (TRF)

● GPCR determination

FRET experiment

Enzyme kinetics determination

ELISA/FIA/TRF-ELISA detection

● Multi label detection

● Ion channel experiment

TR-FRET experiment

BRET experiment

Quantification of DNA, RNA, and protein

technical feature

Fluorescence reading function: (fluorescence and TRF)

Reading board type: 6-hole -1536 hole board

Wavelength selection: 4 monochromators (excitation and emission are dual monochromators respectively)

Excitation light wavelength: 200-800nm

Emission wavelength: 270-840nm

Excitation/emission light broadband: 5nm or 12nm/12nm

Wavelength resolution: 1nm

Light source: High energy Xenon flash lamp

Detector: Photomultiplier Tube (PMT)

Sensitivity: Fluorescence top reading:< 0.4 fmol fluorescein/well,> 6tecades, 384 well plate

Bottom reading:< 0.4 fmol fluorescein/well,> 5.5 decks, 384 well plate

TRF time-resolved fluorescence:< 120amol fluorescein/well,> 6tecades, 384 well plate

Chemiluminescence function:

Reading board type: 6-hole -1536 hole board

Wavelength selection: any wavelength, filter and dual monochromator

Wavelength range: High sensitivity mode 360-670nm, scanning mode 270-840nm

Sensitivity:< 7amol fluorescein/well,> 7decades， flash ATP， 384 well plate

Detector: Dark Current Quantum Counting PMT

Colorimetric reading function:

Reading board type: 6-hole -384 hole board

Wavelength selection: dual monochromator

Wavelength range: 200-1000nm

Broadband: 5nm

Light source: High energy Xenon flash lamp

Detector: Photodiode (PDT)

Linear range: 0-4Abs, (96 well plate) at 450nm,< ±2％

0-3Abs, (384 well plate) at 450nm,< ±2％

Absorption value resolution: 0.001Abs

Accuracy: ＜ ±1％， at 400-1000nm（0-3Abs）

＜±2％， at 200-399nm（0-2Abs）

Accuracy: SD<0.001Abs or CV<0.5%, at 450nm（0-3Abs）

Liquid divider, up to 3, automatic liquid level control

Applicable plate type: 6-384 well plate

Injector: 1ml (standard), 5ml (optional)

Separation volume: 1-1000ul, increment of 1ul (1ml syringe)

Accuracy: ＜ 0.2ul or 2%, 5-1000ul (1ml syringe, 0.40mm pipette tip)

Accuracy:< 2%, 20-1000ul;< 5%, 5-19ul (1ml syringe, 0.40mm pipette tip)

Separation speed: 30 seconds, 96 well plate, 5ul/well, 1ml syringe, 0.40mm separation nozzle

80 seconds, 384 well plate, 5ul/well, 1ml syringe, 0.40mm dispensing nozzle

Incubator: Temperature range: Room temperature+4 ℃ to 45 ℃

Oscillator: Circular oscillation, adjustable speed and diameter

General characteristics:

Measurement speed: 96 well plate, 15 seconds; 384 well plate, 45 seconds; 1536 orifice plate, 135 seconds (when selecting the minimum dynamic interval time)

Spectral scanning speed: ＜ 2S/hole, scanning a span of 100nm in 2nm steps,

Volume: 540mm (W) × 580mm (D) × 500mm (H)



Ordering information:

5250030 Varioskan Flash with research version software

5250040 Varioskan Flash with bottom reading and research version software

5250500 Varioskan LumiSens option, chemiluminescence component

5250510 Dispenser option, Dispenser option