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TSK-GEL series gel chromatographic column
TSK-GEL series gel chromatographic column
Product details
TSK-GEL SW series gel chromatographic column
The packing of TSK-GEL SW series chromatography column is made of rigid spherical silica gel as the matrix, and hydrophilic groups are chemically bonded on its surface through covalent bonds. The packing of TSK-GEL SW series chromatography columns has the necessary properties for high-performance size exclusion chromatography, namely low adsorption and good pore size distribution; Its pH range is 2.5-7.5, and it can use organic solvents that are completely miscible with water, such as acetonitrile, acetone, methanol, or ethanol. The TSK-GEL SW series chromatography column is suitable for analyzing and separating protein and peptide samples, and there are numerous literature reports on other applications. The structure of analytical chromatography columns can be made of glass or stainless steel material.
Types and characteristics of some chromatographic column packing materials in TSK-GEL SW series
stationary phase
Granularity（μm)
Aperture（Å）
Molecular weight of the sample
globulin
glucan
polyethylene glycol
G2000SWXL
5
125
5，000~150，000
1,000~30,000
500~15,000
G2000SW
10，13，20
125
5,000~100,000
1,000~30,000
500~15,000
G3000SWXL
5
250
10,000~500,000
2,000~70,000
1,000~35,000
G3000SW
10，13，20
250
10,000~500,000
2,000~70,000
1,000~35,000
G4000SWXL
8
450
20,000~7,000,000
4,000~500,000
2,000~250,000
G4000SW
13，17
450
20,000~7,000,000
4,000~500,000
2,000~250,000
TSK-GEL PW series gel chromatographic column
The packing material of the TSK-GELPW series chromatography column is a hydrophilic and rigid polymer with excellent chemical and mechanical properties, and a porous spherical shape. It is suitable for analyzing and separating proteins, peptides, polysaccharides, oligosaccharides DNA、RNA、 Water soluble organic polymers and other water-soluble macromolecular samples. The general pH range for the TSW-GELPW system to chromatographic columns is 2-12, and a mixed organic solvent containing 50% can be used as the mobile phase. The operating temperature can reach 80 ℃ (TSK gel G-DNA-PW is 50 ℃). Although most TSK-GEL PW fillers exhibit weak negativity (approximately 5-8 μ eq/mL), they have no effect on the analysis and separation of non-ionic, anionic, and most cationic samples. Because TSK-GELPW packing has a very wide separation range, a more linear calibration curve, and much lower adsorption characteristics than TSK-GELSW chromatography columns, TSK-GEL PW chromatography columns are often used to separate water-soluble synthetic polymers.
Types and characteristics of packing materials for TSK-GEL PW series chromatographic columns
|
stationary phase |
Granularity（μm) |
Aperture（Å） |
Molecular weight of the sample |
||
|
globulin |
glucan |
polyethylene glycol |
|||
|
G2000PW |
10 |
125 |
<5,000 |
~ |
<2,000 |
|
G2500PWXL |
7 |
<200 |
<8,000 |
<3,000 |
~ |
|
G2500PW |
12,17,20 |
<200 |
~ |
~ |
<3,000 |
|
G3000PWXL |
7 |
200 |
500-800,000 |
<60,000 |
<50,000 |
|
G3000PW |
10,17,20 |
200 |
~ |
~ |
<50,000 |
|
G4000PWXL |
10 |
500 |
10,000-1,500,000 |
1,000-700,000 |
<300,000 |
|
G4000PW |
17,20 |
500 |
~ |
~ |
<300,000 |
|
G5000PWXL |
10 |
100 |
<10,000,000 |
50,000-25,000,000 |
<1,000,000 |
|
G5000PW |
17,20 |
100 |
~ |
~ |
<1,000,000 |
|
G6000PWXL |
13 |
>1000 |
<20,000,000 |
500,000-50,000,000 |
<8,000,000 |
|
G6000PW |
17 |
~ |
~ |
~ |
<8,000,000 |
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