Stereoscopic digital display fluorescence module can be matched with various brands of Galileo optical system stereo microscopes, enabling ordinary microscopes to achieve fluorescence function without changing the original optical path of the microscope, making it easy for ordinary microscopes to achieve fluorescence observation function. Adopting a dual color three channel design, it can meet the observation needs of up to 3 colors of fluorescence. It can be equipped with relevant bands including V/B/G/Y/R, and is matched with a digital display screen design to meet the requirements of quantitative experiments, making brightness adjustment more controllable. In addition, the field of view has been expanded, and compared to before, it has higher compatibility and matching with microscopes.