Principle:
By controlling the particle size to achieve physical filtration effect, the impact of substances with a diameter greater than the filter aperture on the system is eliminated.
The significant advantages are:
1. Can be used with columns from all different manufacturers;
2. The filter can be cleaned and reused;
3. One time purchase for long-term use.
Liquid phase detection application

High performance liquid chromatography HPLC detection scheme for doxycycline hydrochloride
-Sample processing procedure-
Accurately weigh an appropriate amount of doxycycline hydrochloride reference substance, add an appropriate amount of 0.01mol/L hydrochloric acid solution, dissolve by ultrasound, and dilute with 0.01mol/L hydrochloric acid solution to a solution containing approximately 1mg doxycycline hydrochloride per 1ml. Shake well, filter through a 0.45 µ m organic system filter membrane, accurately measure 20 µ L, inject into a liquid chromatograph, and record the chromatogram.
-Chromatographic conditions-
Chromatographic column: C18, 4.6 mm*250 mm, Particle size 5 µ m
Mobile phase: Acetonitrile: Acetate buffer solution=25:75
Wavelength: 280nm
Flow rate: 1.0mL/min
Instrument configuration:
Please click to view the image details
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Vertex C18 ColumnFeaturing high surface coverage and complete tail sealing, it is suitable for chromatographic analysis in a wide range of pH values from 1.5 to 10.0...... |
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AP-01P oil-free vacuum pumpIt is a small vacuum pump with a lightweight and beautiful appearance, high efficiency, low noise, and adjustable pressure...... |
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Voltage resistance: 20MP.
Dead volume: 2.8ml.
Filter particles: 1um.
Filtering accuracy: 2-5 μ m
Suitable flow rate: 1-1000ml/min
Supporting pipeline: 1/8 pipeline
Stainless steel, automatic
Liquid phase detection application

High performance liquid chromatography HPLC detection scheme for doxycycline hydrochloride
-Sample processing procedure-
Accurately weigh an appropriate amount of doxycycline hydrochloride reference substance, add an appropriate amount of 0.01mol/L hydrochloric acid solution, dissolve by ultrasound, and dilute with 0.01mol/L hydrochloric acid solution to a solution containing approximately 1mg doxycycline hydrochloride per 1ml. Shake well, filter through a 0.45 µ m organic system filter membrane, accurately measure 20 µ L, inject into a liquid chromatograph, and record the chromatogram.
-Chromatographic conditions-
Chromatographic column: C18, 4.6 mm*250 mm, Particle size 5 µ m
Mobile phase: Acetonitrile: Acetate buffer solution=25:75
Wavelength: 280nm
Flow rate: 1.0mL/min
Instrument configuration:
Please click to view the image details
|
LC-10Tvp high-pressure constant current infusion pumpIt is a multifunctional constant current infusion pump with high flow accuracy, corrosion resistance, and high pressure resistance...... |
|
SPD-10Tvp UV detectorA dual beam monochromatic system composed of imported deuterium lamps, photovoltaic cells, and 1200/mm concave gratings...... |
|
8725i injection valveIt is a six way valve in which the sample is loaded through an embedded needle track in front of the valve...... |
|
Vertex C18 ColumnFeaturing high surface coverage and complete tail sealing, it is suitable for chromatographic analysis in a wide range of pH values from 1.5 to 10.0...... |
|
AP-01P oil-free vacuum pumpIt is a small vacuum pump with a lightweight and beautiful appearance, high efficiency, low noise, and adjustable pressure...... |
|
VI2010 Chromatography WorkstationBreaking through the bottleneck of current domestic chromatography software only having serial port communication, truly realizing USB interface communication...... |






