Live cell super-resolution imaging technology

Nikon's newly launched N-SIM microscope system can achieve twice the resolution of traditional optical microscopes. Using unique optical technology and manufacturing techniques, Nikon's globally renowned Eclipse Ti research grade microscope is equipped with an excellent CFI Apo TIRF 100x oil objective (N.A. 1.49), which is combined with SIM technology authorized by UCSF (University of California, San Francisco) to form a new N-SIM system. N-SIM achieves the industry's highest super-resolution image acquisition speed with a temporal resolution of 0.6 seconds per frame while achieving super-resolution.

|Nearly twice the resolution of traditional microscopes

By combining the UCSF authorized "S structured I illumination M microscopy" with Nikon's renowned CFI Apo TIRF 100x oil objective (N.A. 1.49) N-SIM, almost twice the resolution of traditional optical microscopes has been achieved.

Comparison of Conventional Images and N-SIM Images of Human Vascular Endothelial Cells

|Time resolution of 0.6 seconds per frame, the fastest in the industry

N-SIM provides the industry's highest super-resolution image acquisition speed, achieving a time resolution of 0.6 seconds/frame for 2D SIM and 1 second/frame for 3D SIM, enabling effective live cell imaging.

Dynamic image of mitochondria labeled with red fluorescent probe (3D SIM, 1 second/frame)

|Multiple imaging modes

|TIRF-SIM/2D-SIM mode

The newly developed TIRF-SIM illumination technology provides higher resolution for total internal reflection fluorescence observation (TIRF) compared to traditional TIRF microscopes, which can obtain more detailed information near the cell membrane.

TIRF-SIM image Traditional TIRF image Plasma membrane of B16 melanoma cell labeled with YFP
Objective: CFI Apochromat TIRF 100x oil (NA 1.49)
Photographed with the cooperation of: Dr. Yasushi Okada, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN

|3D-SIM mode

Two 3D modes can be used. The "Slice 3D-SIM" mode is used for single-layer super-resolution imaging with a Z-axis resolution of 300nm; The "Stack 3D-SIM" mode is used for sequential multi-layer super-resolution imaging of thick samples, with higher contrast compared to the former.

Slice 3D-SIM image, ordinary microscope image Bacillus subtilisbacterium stained with membrane dye Nile Red (red), and expressing the cell division protein DivIVA fused to GFP (green).
The superior resolution of N-SIM system allows for accurate localization of the protein during division.
Photos courtesy of: Drs. Henrik Strahl and Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle University Stack 3D-SIM image (3D reconstruction) maximum transmission image Mouse keratinocyte labeled with an antibody against keratin intermediate filaments and stained with an Alexa 488 conjugated second antibody.
Photos courtesy of: Dr. Reinhard Windoffer, RWTH Aachen University

|Simultaneous dual wavelength super-resolution imaging

Use the optional dual camera component * to link two EMCCD cameras to the microscope. Simultaneous dual wavelength super-resolution imaging can be achieved using 488nm and 561nm excitation light.*Andor Technology Ltd

Dual Camera Imaging Adapter (N-SIM)*The appearance of the product shall be based on the actual product.

Growth cone of NG108 cell expressing GFP-LifeAct (F-actin, green) and mCherry-tubulin (microtubules, red)
Photos courtesy of: Dr. Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST)

|5 Laser multi-color imaging

The LU5 N-SIM 5 laser station can be equipped with up to 5 lasers providing five wavelengths for true multi-color super-resolution imaging. The multi-color super-resolution imaging capability is a key technology for studying the dynamic interactions between multiple proteins at the molecular level.

Mid mitotic human U2OS cellsMark green: Kinetochore protein CENP-B, red: Alpha tubulin, blue: DNAPhoto courtesy of: Dr. Alexey Khodjakov, Wadsworth Center, Albany NY

|Special objective lens for super-resolution microscope

Super Resolution lenses are specially developed for the latest super-resolution applications. Using the latest optical design and carefully selected, it has the least optical phase difference and color difference, ensuring the best optical performance.

CFI SR Apochromat TIRF 100x oil

CFI SR Plan Apochromat IR 60x WI