Mettler pH electrode InPro 2000i SG/450/9816

Specification - pH Sensor InPro 2000i SG/450/9816

Mettler pH electrode InPro 2000i SG/450/9816

9848: Suitable for biochemistry, media containing proteins, organic solvents, and low temperatures
9816: Suitable for chemical processes, slow electrolyte flow rate 9830: Suitable for media containing a large amount of organic solvents 9823: Classic electrolyte, fast electrolyte flow rate, helps with membrane cleaning

Online monitoring of fermentation process: The penicillin fermentation of Weiqida Pharmaceutical adopts a production method of small amount and multiple feeding Allow the matrix that is prone to inhibition, suppression, and restriction to flow slowly To maintain the optimal concentration Especially for the flow of glucose, even small fluctuations beyond the optimal concentration range All of them may cause serious obstruction or restriction Slow down or stop the rate of biosynthesis. Currently The detection of sugar concentration is still difficult to conduct online monitoring The flow of glucose cannot be controlled based on sugar concentration. At the same time Penicillin is a secondary metabolite produced by Aspergillus flavus during its growth process In the production process, the secondary metabolism rate of penicillium is regulated The main control basis is the fermentation unit, and the main control methods include pH regulation, dissolved oxygen regulation, tank temperature control, and feed control. The fermentation process of penicillin requires precise pH monitoring to help adjust feeding strategies. Weichida Pharmaceutical uses the Mettler Toledo online pH measurement system to help achieve precise measurement and regulation of this process The speed of glucose flow acceleration is determined based on changes in pH value Optimize the automated feeding process. The optimal pH value for penicillin fermentation is generally considered to be around 6.5 It can be slightly higher or slightly lower But efforts should be made to avoid pH values exceeding 7.0.
Because penicillin is unstable under alkaline conditions and easily accelerates its hydrolysis. A low sugar addition rate is difficult to neutralize the alkaline compounds produced by the metabolism of ammonia or other physiological alkaline substances in protein metabolism Causing an increase in pH value Excessive flow acceleration rate can lead to the accumulation of acidic intermediates and a decrease in pH value. During the production process, pH monitoring can help control the stability of acid and alkaline materials. Dissolved oxygen concentration is another important factor affecting the fermentation process of penicillin. Penicillin requires different dissolved oxygen values during different fermentation cycles Too high will increase carbon source consumption and affect important secondary metabolism If it is too low, it will cause the mycelium to self dissolve due to hypoxia. Shanxi Weiqida Pharmaceutical has also achieved automated monitoring in dissolved oxygen control The InPro 6800i electrode of the Mettler Toledo online dissolved oxygen system is directly inserted into the fermentation tank to measure real-time changes in the fermentation broth And provide accurate dissolved oxygen value indication through M400 transmitter As an important reference indicator for glucose flow control Thus ensuring a dynamic balance between oxygen transfer and oxygen consumption. In theory, it is generally assumed that the biomass at every point in the fermentation broth is the same But the dissolved oxygen content is different when only considering pressure factors The actual dissolved oxygen at the bottom of the tank is higher than that at the top, so the selection of the installation point for measuring fermentation tanks is also very important.

Online monitoring of extraction process
After filtering the penicillin fermentation broth Generally, extraction method is used for purification Weiqida Pharmaceutical also adopts a similar method Extraction is achieved through a disc centrifuge. The downstream extraction process of Vicida penicillin is also equipped with a Mettler Toledo online pH measurement system To achieve efficient automated extraction through high-precision online monitoring. Firstly, add dilute sulfuric acid solution to the penicillin fermentation filtrate to adjust the pH to around 2.0 Add butyl acetate and demulsifier for extraction The operating temperature is generally set at 10 ℃~15C. After extraction, the organic phase is washed by centrifugation Perform the second step of extraction The washed organic phase and alkaline water were subjected to three-stage continuous countercurrent back extraction using a centrifuge at low temperature to adjust the pH value to 6.6-7.2. This purified the target substance from the organic phase to the aqueous phase. This extraction method has the advantages of fast mass transfer speed, short production cycle, easy continuous operation, automatic control, high separation efficiency, and large production capacity, especially in improving production efficiency through automation control. last. The obtained reverse extraction solution is subjected to azeotropic crystallization with butanol and dried Further obtain refined finished products. Weiqida Pharmaceutical further ensures the efficacy, purity, and subsequent crystallization process speed by controlling the pH value of the solution during the penicillin extraction process It saves production costs, improves production efficiency, and ensures high-quality products.