LCMS-9030 adopts a new patented technology to achieve high resolution and excellent accurate quality data. Provide reliable information for the identification and molecular formula derivation of unknown compounds. Efficient ion introduction units, quadrupole rods, and collision cell technologies can achieve high-sensitivity detection of trace compounds. Unique UFgrating and iRefTOF technologies enable ultra fast acceleration of ions into the UF LightTube, where they fly in this ideal reflection field and reach the detector. Combining high-speed data acquisition system to achieve high-throughput analysis.
High efficiency quadrupole technology
UFaccumulation ™
Ion accumulation is performed before entering TOF, perfectly synchronized with TOF pulse time and data acquisition cycle, maximizing sensitivity.
UFgrating ™
Shimadzu's world-class manufacturing capabilities enable ion accelerating electrodes to have high mechanical strength. The grid electrode is capable of withstanding the high voltage required for ultrafast ion pulses.
Funnel MCP
The design of non-destructive microchannel plates maximizes sensitivity.
UF-FlightTube ™
Quality stability is an important prerequisite for quality accuracy. UF FlashTube technology precisely controls the temperature of the flight tube, eliminating the need for frequent calibration and achieving higher analysis efficiency and throughput.
iRefTOF™
This technology provides an ideal electrostatic field that conforms to theoretical calculations. The reflector is made up of carefully manufactured flat electrode stacks that redirect ions without affecting resolution and sensitivity.
Excellent performance, easy implementation
Excellent quality measurement accuracy
For high-resolution accurate mass spectrometry (HRAM), mass determination accuracy is the most critical performance and the foundation of all applications. The quality determination accuracy of LCMS-9030 can provide unparalleled high confidence for unknown substance identification and maintain long-term stability.
The excellent quality measurement accuracy of LCMS-9030 is attributed to its intelligent temperature control system and UF FlashTube technology, which can accurately eliminate the influence of internal and external environmental temperature changes. LCMS-9030 brings customers a brand new experience in using HRAM, more reliable results, easier operation, and longer continuous testing of more samples.
Stability of Quality Measurement Accuracy
The intelligent temperature control system can ensure that the accuracy of quality measurement remains stable when the laboratory temperature changes. To illustrate this point, after one calibration, the measured mass changes of different samples (mass range from 150 Da to 1700 Da) were continuously measured, and the laboratory temperature fluctuated between 25 ° C and 28 ° C. From the experimental results, it can be seen that LCMS-9030 does not require additional mass correction during continuous 60 hour testing, and the measurement error of all compounds does not exceed 1 ppm of the theoretical mass.
Accuracy of mass determination within a wide concentration range
The high sensitivity of LCMS-9030 and the high selectivity brought by the accuracy of mass determination over a wide concentration range have opened up a new foundation for quantitative analysis. Real ion counting ensures that all measurement results during the entire chromatographic peak elution process are within a narrow extraction ion chromatography (XIC) window. As shown in the figure below, in the quantitative analysis of Verapamil, even at a sample size of 10 fg, the mass determination error is less than 1 ppm, and all data points are within the 5 ppm XIC window. Thus achieving accurate, reproducible, and highly selective quantitative analysis. In addition, the stability of the quality determination accuracy of LCMS-9030 allows the same XIC setting to be easily used for sample analysis of a series of different batches.
Conc. (pg/mL)
Calculated conc. (pg/mL)
Area RSD (%, n = 3)
Accuracy (%, n = 3)
500
523.3
1.93
104.67
100
96.3
3.78
96.50
10(LLOQ)
10.3
5.31
100.50
Averaged Mass Spectrum at Each Concentration
Accuracy of quality measurement under different acquisition modes
MS/MS mass spectrometry is a key tool for the structural analysis of unknown compounds, and the convenience of data analysis directly depends on the accuracy of mass determination under MS/MS mode collection. Thanks to the collision cell technology that can generate high abundance fragment ions, LCMS-9030 has the same high-quality determination accuracy as MS mode in MS/MS mode, which is ideal for structural analysis. The MS/MS structural analysis results of cyclosporine are shown in the following figure. It can be seen that the ion mass errors matching the assumed structural fragments are all less than 1 mDa. In addition, the number of molecular formula predictions for m/z 567.3860 with different allowable errors further indicates that the accuracy of mass determination by MS/MS has a direct impact on the correctness of the results.
MS/MS Spectrum of Cyclosporine
Mass Error of Product Ions
Formula Prediction Results form/z567.3860
Selective high-resolution and accurate mass spectrometry
The high-resolution and accurate quality data of LCMS-9030 can distinguish compounds with the same nominal mass. As shown in the figure below, when analyzing 10 ng/ml of triazolam and etizolam and their metabolites in whole blood, 4-hydroxytriazolam, α - hydroxytriazolam, and α - hydroxyetizolam have the same nominal mass, but even if chromatographic separation is incomplete, each compound can be separated and quantitatively detected without interfering with each other. Another pair of compounds with the same nominal mass are Triazolam and Etizolam, and their detection results further demonstrate that LCMS-9030 has the ability to distinguish mass differences of only 0.0267 Da, thereby improving quantitative selectivity.
LCMS-9030 Mass Chromatogram of Etizolam, Triazolam, and Metabolites Spiked at 10 ng/mL in Whole Blood.
Inherited from the design of Shimazu triple quadrupole
The method transfer between LCMS-9030 and Shimadzu triple quadrupole mass spectrometry is very simple and effective, because Shimadzu's LCMS products share relevant technologies in heating ion sources, ion transport focusing, and collision cells. This means that key method parameters such as ESI source conditions, transmission lens voltage, and collision energy are compatible between the two systems. LCMS-9030 is developed based on the high-sensitivity ion technology of triple quadrupole mass spectrometry LCMS-8060, and can produce similar high-quality mass spectra.
In order to make data dependent acquisition mode (DDA) settings easy, LCMS-9030 adopts automatic collision energy (CE) distribution function to achieve effective fragmentation. The automatic collision energy distribution function enables users to obtain high-quality MS/MS spectra even when the optimal collision energy is unknown. In the following example, the QTOF spectrum obtained using the automatic CE distribution function was compared with a series of merged spectra of different collision energies obtained on LCMS-8060. The experimental results demonstrate good consistency between the two, which also proves that LCMS-9030 can obtain high-quality mass spectra in DDA mode.
