Based on laser confocal scanning microscopy system - fluorescence imaging, fluorescence intensity scanning imaging, fluorescence lifetime scanning imaging, fluorescence spectrum scanning imaging

The new generation of Fast Fluorescence Lifetime Imaging (FastFLIM) system provides sensitivity at the single-molecule level, with a standard detection wavelength range of 350-1050nm; Lifespan range: 100ps-100ms; Used in disciplines such as chemistry, nanotechnology, energy, biology, etc., for the mechanism research of single-molecule dynamics, live cells, micro area imaging, morphology, energy level structure, and energy transfer characteristics. The testing speed is up to 5fps (512 × 512), with imaging ranging from 1 × 1 to 4096 × 4096. Visual Phasor plots are used for fluorescence lifetime direct reading of semicircles, and batch fluorescence lifetime imaging data processing has entered the era of direct reading; Mirror scanning is suitable for lifetime imaging at all time scales. Live cell workstation and temperature testing can be selected;

Main function description:

Laser confocal fluorescence intensity imaging LCM;

Fluorescence lifetime imaging FLIM, phosphorescence lifetime imaging PLIM;

Upconversion fluorescence (lifetime) imaging, rare earth luminescence (lifetime) imaging, delayed fluorescence (lifetime) imaging;

Fluorescence fluctuation imaging FFS (FCS, FCCS, PCH, N&B, RICS, FLCS), FLIM-FRET imaging;

Single quantum dot luminescence (lifetime) imaging, single-molecule and single-molecule fluorescence resonance transfer imaging, including alternating excitation PIE imaging;

Steady state and transient polarization imaging;

Micro area fluorescence spectroscopy acquisition and spectral imaging;

Anti beam testing;

Prof FLIM system selected by Joseph R. Lakowicz laboratory

Digital frequency domain technology DFD-FLIM (FastFLIM) and time domain technology TD-FLIM (TCSPC) imaging technology;

Real time direct reading to obtain fluorescence lifetime values and trends, as well as FRET efficiency distribution;

Choose a detector with a wavelength range of 300-1600nm and 2-4 channels for imaging, FLIM-FRET；

Upgraded wavelength free AFM can be used to achieve synchronous testing of same area morphology and FLIM;

Ultraviolet visible infrared excitation wavelength, single wavelength or supercontinuum laser; 266nm-1300nm laser is optional; PIE function is optional; White light laser can be selected;

Single photon or two-photon lasers;