characteristic

• Easy to operate performance
  At present, even in small and medium-sized laboratories, the use of multiple HPLC machines by one person is increasing. Primaide's simple design and easy maintenance of pre installed components make it easy for beginners to use.

• Excellent basic performance
  The requirements for accuracy, reproducibility, and stability of HPLC are increasing day by day. Primaide not only meets the limit requirements, but can also expand its application range with high-performance DAD (Diode Array Detector), automatic sampler cooling unit, and other components.

• Reliable durability performance
  Primaide upholds the highly acclaimed durability of Hitachi HPLC. Balancing performance and durability, each instrument fully meets Hitachi's strict quality standards.

System Characteristics

• Creating a refined and stylish system appearance through the built-in selection of accessories
  The main optional components such as degassing device, gradient unit, and sample cooling unit can be built into the host, and even if optional components are added, they do not occupy additional space.

• Improve operability and maintainability through component pre installation
  All modules can be operated from the front, and piping is also concentrated on the front, making it easy to replace chromatography columns and ensure smooth operation of the flow system. The lamp or circulation pool can be replaced from the front, taking into account maintenance.

• All modules are equipped with leakage sensors
  When there is a leakage in the flow system, the leakage sensors configured in each module can sense and automatically alarm, improving safety and reliability.

• Control through system management software
  All modules can be controlled through system management software without the need for an operation panel. When using a single module, the UI panel (optional accessories) is very convenient.

• Communication using e-Line and USB interface
  Compared to traditional D-Line, D-Line communication is more advanced by interconnecting various modules, centralizing all communication, digitizing signals, and improving communication reliability. The connection with the PC adopts a highly universal USB interface, and the PC does not require any special boards.

• GLP function
  The detector host is equipped with a mercury lamp, which uses its characteristic spectral line (254 nm) to automatically calibrate the wavelength in the ultraviolet region (built-in in Primaide 1410UV/1430DAD). Each module has rich quality assurance information to improve data reliability.

Analysis of catechins

[Standard Sample]
GC: (-) - Epicatechin gallate (thermal isomer) EGC: (-) - Epigallocatechin gallate C: (-) - Catechins EGCG: (-) - Epigallocatechin gallate EC: (-) - Epicatechins GCG: (-) - Epicatechin gallate (thermal isomer) ECG: (-) - Epicatechin gallate CG: (-) - Epicatechin gallate (thermal isomer) Caffeine ： Coffee because

Module lineup

1110 pump

Inheriting the tradition of Hitachi HPLC pumps, a pump developed for routine analysis.
Maintaining stable high performance in daily measurement work is a powerful assistant for your analytical work.

• Improve the precision of flow rate
  Hitachi liquid phase pumps have always used double speed control to suppress liquid pulses. The Primaide 1110 pump can monitor pressure fluctuations in real time and set the double speed range of the plunger at the optimal time to effectively suppress liquid pulses.

• Improve the reproducibility of retention time in gradient analysis
  The injection time of Primaide 1210 automatic sampler is synchronized with the gradient cycle time of the pump, and the retention time of gradient analysis has excellent reproducibility and high analysis accuracy.

1210 automatic sampler

The Primaide 1210 automatic sampler adopts a direct injection method, especially suitable for small and valuable samples.

• Adopting direct injection method to save sample volume and improve analysis speed
  Adopting direct injection method, the injection needle is a part of the flow path, and the sample sucked into the injection needle can be fully injected into the flow path, reducing sample consumption and playing an advantage in the determination of small and valuable samples.
  Compared to the injection method using a quantitative loop, the injection steps are simpler, the injection cycle can be shortened by half, and the measurement speed can be improved, which is helpful for high-throughput analysis.

• Realize high-precision analysis by improving reproducibility
  The driving part of the injector used to control the sample injection volume adopts a high-precision stepper motor, and the injection time is synchronized with the gradient cycle time of the pump, improving the reproducibility of the injection volume and the reliability of the analysis results.

1310 column temperature box

Primaide 1310 column temperature box comes standard with cooling function. The standard preheating function achieves sharp peak shapes, fully utilizing the performance of the chromatographic column.

• Preheating method to achieve sharp peak shape
  By using a preheating module with excellent thermal efficiency, the symmetry and sharpness of chromatographic peaks are improved, unaffected by changes in ambient temperature, and the stability of analysis results is achieved. The chromatographic column is heated by air circulation for easy replacement.

• Standard cooling function
  Realize temperature control from 5 ℃ to 65 ℃ (room temperature of 20 ℃).

1410 UV detector

Low noise, low drift optical system. The lamp and circulation pool are both front mounted, easy to replace.

• Low noise, high sensitivity
  The noise level of Primaide 1410 UV detector is below 0.6 × 10-5AU, making it particularly suitable for analyzing trace components.

• High response and low noise support for high-speed analysis
  A response time of 0.05 seconds can also achieve low noise, true detection of sharp peak shapes, and high-speed and high-sensitivity analysis.

• Stability that is not easily affected by environmental temperature changes
  We are committed to the constant temperature design of optical systems, which can provide stable and highly reproducible analysis results regardless of the customer's laboratory environment, day night temperature, or seasonal temperature changes.

• Automatic wavelength calibration in the ultraviolet region using a built-in mercury lamp
  The 254nm characteristic spectral line of the mercury lamp can be used to verify the UV wavelength range commonly used in HPLC. Combined with the characteristic spectral lines of D2 lamp, three wavelengths can be verified, thus enabling highly reliable analysis over a wide wavelength range.

1430 diode array detector (DAD)

The sensitivity of the Primaide 1430 DAD detector is comparable to that of a UV detector, and it also has three-dimensional system specific functions such as spectrum acquisition, multi wavelength simultaneous detection, peak purity detection, and spectral database retrieval.

• Support high-sensitivity analysis
  By adopting a new optical system and improved software, low noise (≤ 0.5 X 10-5AU (250 nm/600 nm)) has been further achieved. The sensitivity of the DAD detector is not good "- there is no need to be confused about this - traditional concept.

• Structure that is not easily affected by temperature fluctuations in the measurement environment
  By adopting a large lamp chamber, the stabilization of the light source has been achieved. The splitter is separated and insulated from the lamp chamber, only cooling the lamp chamber and improving its adaptability to environmental temperature changes. Built in temperature sensor, using a fan that synchronizes with temperature changes to control air volume, further reduces temperature fluctuations inside the instrument, ensuring low noise and high stability, and guaranteeing the stability of analysis results.

• Hyperspectral resolution
  Configure a 1024 bit diode to achieve high spectral resolution (0.78 nm/1 element). By using gratings as spectrometers, the same spectral resolution can be obtained from the ultraviolet to visible light regions.

• Realize high-speed analysis through digital noise correction processing
  A shorter data collection interval (0.05 seconds) can also achieve low noise, and sharp peak shapes can also be obtained through low noise in high-speed analysis.

• Equipped with automatic wavelength calibration function
  Perform automatic wavelength calibration in the ultraviolet region using a built-in mercury lamp.

Organizer

The organizer can be placed in multiple solvent bottles

The following solvent bottles can be placed simultaneously in the organizer.

Organizer doubles as power supply module

The organizer is also a power module that supplies power to one pump, one automatic sampler, one detector (one UV detector, one UV visible detector, one diode array detector, or one differential refractive index detector), and one IFC interface board.

1440 fluorescence detector

The Primaide1440 fluorescence detector has high sensitivity and is particularly suitable for the analysis of trace samples.

• high sensitivity
  The optical system adopts a three-dimensional optical axis configuration optical design, Hitachi's unique condenser lens, small volume flow cell, and optimized transmission light monitoring technology to reduce the loss of light energy.

• Variable slit fluorescence detector
  The slit of the emission monochromator can be selected between 15 nm and 30 nm, and using a 30 nm slit can be used for higher sensitivity analysis.

• Automatic wavelength correction
  The 254 nm characteristic spectral line of mercury lamp was used to verify the wavelength in the UV range commonly used in HPLC, with high wavelength accuracy.

1320 post photochemical derivatizer

The Primaide 1320 post column photochemical derivatizer can easily achieve trace detection of aflatoxins in samples.

• Has excellent photochemical derivative properties
  Using high-quality imported components and an internally designed heat dissipation system to ensure long-term stable operation of the analysis system.
  The specially designed internal optical path and reaction tube further improve the derivative efficiency and detection sensitivity.

• Equipped with LAMP OFF function
  Equipped with a light source monitoring indicator light, the light source status can be checked at any time.
  Equipped with LAMP OFF function, it can automatically turn off the lights after the sequence runs, saving the lifespan of the light source.