Molecular fluorescence has attracted great attention in the field of analysis and testing due to its high sensitivity and selectivity. However, due to two issues that affect the linear testing interval of quantitative testing, fluorescence is often used for qualitative purposes, such as fluorescent biomarkers. The problem is, 1 How to avoid the interference of stray light from excitation light in low concentration samples, and how to avoid the absorption effect of fluorescent samples in high concentration samples, both of which can lead to quantitative results deviating from the linear range at lower and higher concentrations.

AquaLog - the world's first spectrometer to simultaneously measure fluorescence (3D fluorescence 3D-EEM) and ultraviolet spectroscopy, and the first complete fluorescence quantification device. Effectively eliminating optical noise interference from the excitation source and solving the self absorption effect of high concentration samples; Multiple luminescent substances in the same sample can be quantitatively tested simultaneously; Greatly expanding the quantitative application field of fluorescence spectroscopy (single wavelength intensity peak area peak volume quantification).

It can be effectively used for 3D fluorescence 3D-EEM quantitative testing, such as CDOM quantitative testing required for water analysis, which can provide quantitative reference data for chlorine addition in water plants. The data and TOC have referenceability.

The instrument comes with excitation source correction, excitation spectrum correction, emission spectrum correction, internal filter correction, dark noise correction, 1st and 2nd level sharp scattering zeroing, and Raman peak difference reduction zeroing calculation when it leaves the factory.

The AquaLog 3D fluorescence spectrometer adopts a dual grating excitation monochromator and fast multi-channel detection (S/N up to 20000:1), which can instantly complete the acquisition of 2D full spectrum data; At the same time, it can eliminate sharp scattering to zero. These designs meet the needs of fluorescence research related to water quality analysis and quantitative and qualitative analysis of complex mixed organic compounds, such as rapid data acquisition of three-dimensional fluorescence; Transmission/absorption photometric values are used for reabsorption correction; Quinine sulfate units are used as fluorescence intensity standards, etc.

The real living environment forces people to focus more on water quality related topics such as drinking water management; Pollution from reservoirs, lakes, rivers, and oceans; Soil erosion, red tide, oil leakage; Industrial and agricultural insecticides, fertilizers, etc. Fluorescence spectroscopy has become an important tool for water quality analysis due to its excellent sensitivity and high selectivity. The AquaLog 3D fluorescence spectrometer combines absorption measurement and 3D fluorescence spectroscopy technology, with fast operation, high detection sensitivity, and the ability to obtain more signals. Its detection speed has been increased by 100 times. Absorption measurement can make data stable and accurate, and absorption measurement and fluorescence scanning can be performed simultaneously, saving testing time. You can choose fiber optic accessories, liquid-phase flow cell accessories, etc.

Technical specifications of Aqualog EEM three-dimensional fluorescence spectrometer

1. Function: Three dimensional fluorescence quantitative analysis, EEM-3D spectrum/absorption spectrum emission spectrum absorption spectrum and fluorescence kinetics;

2. Excitation source: 150W continuous xenon lamp; Equipped with a rear reflective curved mirror;

3. Excitation monochromator: The dual grating excitation monochromator is equipped with an automatic elimination filter wheel;

4. Excitation grating: 1200g/mm, 250nm blazed wavelength.

5. Excitation spectral bandwidth: 5nm

6. Excitation wavelength accuracy: ± 1nm

7. EEM excitation wavelength range: 200-850, emission range: 250-850nm;

8. 3D spectral scanning mode: from long wavelength to short wavelength, reducing the probability of sample bleaching by ultraviolet light.

9. Signal to noise ratio: greater than 20000:1 (RMS, 350nm EX, water Raman S/N);

10. Emission monochromator: CCD emission spectrometer; Grating specifications: engraved line density of 405g/mm, shining wavelength of 250nm; (Extended 285 gr/mm; 350 nm blaze)

11. Emission detector: 1024 × 64 TE cooled backlit CCD, 0.41nm/pix (0.82, 1.64, 3.28nm/pix)

12. Built in real-time internal absorption correction detection scan;

13. Full spectrum readout time of emission spectrum: 5 ms

14. EEM full spectrum readout time: 30s

15. Absorption measurement wavelength range: 230-800nm

16. Software calculation function: zeroing of 1st and 2nd order scattering spectra and reducing Raman peak difference; All Origin 8.5 features.

17. System calibration functions: dark noise zeroing calibration, real-time calibration of excitation sources, calibration of excitation spectra, calibration of emission spectra, and real-time calibration of internal absorption.

18. Built in comprehensive performance verification testing:

NIST fluorescence standard reference material for spectral calibration and correction SRM: 294029412422943

Sterna standard reference material used for calibration of quinine sulfate fluorescence emission spectrum RM-QS00

NIST absorption standard for UV visible spectrophotometer SRM931g

Starna standard for UV visible spectrophotometer RM-06HLKI

Raman signal-to-noise ratio evaluation of water

19. Quantification: Built in normalization of fluorescence intensity using quinine sulfate units and Raman scattering units.