EMS GloQube®The glow discharge treatment instrument is a new type of compact and easy to operate instrument, which is particularly suitable for the modification and surface cleaning of copper mesh and support film during sample preparation in the electron microscope room. It is also suitable for the surface hydrophilicity, hydrophobicity, and charge treatment of biological laboratory slides and other materials.

EMS GloQube®Dual chamber design, one for surface hydrophobic and hydrophilic modification treatment; One is used for surface cleaning treatment, using reagents such as methanol.

In order to prevent accidents, high-voltage electrical wires are shielded; The plasma current can be adjusted by adjusting the vacuum level. In order to maximize the efficiency of the instrument, argon gas is used as the working gas during glow discharge.

An automatic switching valve is installed between the two chambers to prevent cross contamination. Convenient loadingTEMThe carrier network and carbon support membrane samples are equipped with height adjustable sample tables in each chamber, which can be clamped2a25X75mmThe glass slide. Safe injection of argon gas, using membrane sealed bottles, reagent processing is very safe, simple push and puncture loading. Quickly input parameters and store programs, which can achieve“Chamber selection, creation, editing, or calling programs”Function, real-time display of program running status, with help menu and running log.

Like hydrophobic (oleophilic) propertiesTEMAfter gas glow discharge treatment in an air atmosphere, the surface of the carbon supported membrane is negatively charged and hydrophilic, making it easy to disperse in aqueous solution. withTEMThe popularization of electron microscopy applications and the increase in workload,TEMThe quality of the carrier network and supporting membrane has become more important than ever before. Except for ordinary ones with carbon support filmTEMIn addition to copper mesh applications, it is also widely used for adhering carbon films, proteins, antibodies, nucleic acids, and positively charged protein molecules to nucleic acids(as:Ferritin, cytochromec)Waiting for various different requirementsTEMSample pretreatment. It can achieve four different processing results:

1）Hydrophilic and negatively charged treatment

Glow discharge is used to change the carbon film, Fanghua film, andTEMThe recognized method for surface characteristics of carbon supported membranes. Carbon is naturally hydrophobic, and short glow discharge treatment can change its surface properties to hydrophilic.

2）Hydrophilic and positively charged treatment

Cannot be obtained solely through glow discharge“Hydrophilicity and positive charge”Usually, air atmosphere glow discharge is performed first, followed by magnesium acetate(Magnesium acetate)or0.1%（W/V）Polylysine solution treatment. Commonly used to enhance the adhesion of nucleic acids to carbon films.

3）Hydrophobic and positively charged treatment

The carbon film treated in alkylamine vapor carries a positive charge. Deposition of polymers leads to changes in membrane size. Suitable for negatively charged macromolecules, it can be used to chemically adsorb various biomolecules including proteins, antibodies, and nucleic acids.

4）Hydrophobic and negatively charged treatment

Carbon films treated in a hydrocarbon reducing atmosphere (usually using methanol) can produce hydrophobicity–veOn the surface, it can strongly adsorb positively charged macromolecules such as ferritin and cytochromeCIsoprotein family (related to mitochondrial inner membrane).

Features and advantages:

1）Dual chamber design

2）Hydrophilic, hydrophobic, and positively/negatively charged modes

3）Fully automated operation, high work efficiency

4）Computer touch screen control

5)）Convenient sample loading and unloading

6）Controlled gas loading method to prevent sample disturbance and damage

7）The automatic switching of valves between the two chambers prevents cross contamination from occurring

8）Small independent system, compact structure

9）The results are consistent and reliable, with good reproducibility

Main technical specifications:

1）Plasma current:1-40 mA

2）Power Supply:30 W

3）Maximum output voltage:800V

4 )Sample table size:125X100mm

5）Running time:1-600S

6）Working vacuum range:0.1to1 mbar

7）Chamber size:100mmWX100mmHX127mmD

Instrument size:136mmHx364mmD

Weight:19.5kilogram