Innovative top imaging platform

ECLIPSE Ti2 achieves an unprecedented 25mm field of view (FOV), completely changing the way you observe. With its groundbreaking large field of view, Ti2 can freely utilize the sensor area of large target CMOS cameras and significantly improve data acquisition.

The Ti2 stage, tailored for ultra-high resolution imaging systems, exhibits extremely stable and unbiased performance, while its unique hardware triggering function allows for easy handling of the most demanding high-speed imaging experiments. The unique intelligent module of Ti2 can collect internal sensor data, guide users to complete the imaging process, and prevent misoperation. In addition, during data collection, the status of each sensor will be automatically recorded, ultimately achieving high-quality imaging and improving data reproducibility.

Combined with Nikon's powerful image acquisition and analysis software NIS Elements, Ti2 is undoubtedly a revolutionary leader in the imaging field.

|Breakthrough Big View

As research trends move towards large-scale, system level approaches, the market's demand for faster data collection and higher throughput capabilities is increasing day by day. The development of sensors for large target area cameras and the improvement of computer data processing capabilities have driven this research trend. With an unprecedented 25mm field of view, Ti2 provides a higher level of measurability, allowing researchers to truly maximize the role of large target area detectors and ensure that its core imaging platform adapts to future needs as camera technology continues to rapidly develop.

Neuronal microtubule staining (Alexa Fluor 488); Shoot with a CFI Plan Apo lambda 60x objective lens and DS-Qi2 camera. The above picture shows the traditional perspective, and the following picture shows the brand new perspective of Ti2.
The photo was provided by Josh Rappoport from the Nikon Imaging Center at Northwestern University;
The specimens were provided by S. Kemal, B. Wang, and R. Vassar from Northwestern University.

|Bright field lighting with a wide field of view

High power LED provides bright illumination within the large field of view of Ti2, ensuring clear and consistent results under strict requirements such as high magnification differential interference (DIC). By adopting a compound eye lens design, Ti2 can provide uniform illumination from one side to the other. This is highly beneficial for quantitative high-speed imaging and large image stitching.

High power LED lighting fixture

Built in compound eye lens

We have designed a specialized compact epifluorescence illumination device for large field imaging. It is equipped with a quartz material compound eye illumination lens and can provide a wide spectrum of high transmittance, including ultraviolet. The large-sized fluorescent filter block with hard coating can provide images with a large field of view while ensuring high signal-to-noise ratio.

Large field of view falling fluorescent illumination device

Large size fluorescent filter block

|Large diameter observation light path

Observe the expansion of the optical path diameter, so that the imaging port can achieve a field of view of 25. The large field of view obtained from this can capture an area approximately twice that of traditional lenses, allowing users to fully utilize the optimal performance of large target sensors such as CMOS detectors.

Expanded cylindrical mirror

A super large imaging port with a field of view of 25

|Objective lens for large field imaging

The objective lens with excellent image flatness ensures high-quality images from one side to the other. Fully utilizing the maximum potential of OFN25 objective lens can greatly accelerate the data acquisition process.

|Camera for high-throughput data acquisition

The high-sensitivity monochrome camera DS-Qi2 and high-speed color camera DS-Ri2 have CMOS sensors with dimensions of 36.0 x 23.9 mm and 16.25 million pixels, which can fully utilize the best performance of Ti2's 25mm large field of view.

D-SLR camera technology optimized for microscopes

DS-Qi2

DS-Ri2

|Excellent Nikon optical components

Nikon's high-precision CFI60 infinity optics are designed for various complex observation methods and have been widely praised by researchers for their excellent optical performance and solid reliability.

|Cut toe difference

Nikon's unique apodization objective lens uses a carefully selected amplitude filter to significantly enhance contrast and reduce halo artifacts, providing fine high-definition images.

Toe cutting phase plate integrated into APC objective lens

BSC-1 cells captured using CFI S Plan Fluor ELWD ADM 40xC objective lens

|External difference (Ti2-E)

The electric external phase difference system combines phase difference with fluorescence imaging by avoiding the use of phase difference objectives, without affecting the efficiency of fluorescence. For example, liquid immersion objectives with high numerical aperture (NA) can be used for phase contrast imaging. Through this external phase difference system, users can easily combine phase difference with other imaging modes, including weak fluorescence imaging such as TIRF and optical tweezers.

Epifluorescence and external phase contrast images:
PTK-1 cells labeled with GFP alpha microtubule protein were photographed using a CFI Apo TIRF 100x Oil objective lens provided by Dr. Alexey Khodjakov, Scientific Researcher VI/Professor at Wadsworth Center

|DIC (Differential Interference Difference)

Nikon's highly acclaimed DIC optics provide uniform, fine, high-resolution, and contrast images at various magnifications. DIC prism is specially customized for each objective lens, providing the highest quality DIC images for each specimen.

Install DIC prisms that match each objective lens in the objective disc

Differential Interference Difference (DIC) and Epifluorescence Images:
Neuron images with a field of view size of 25mm (DAPI, Alexa Fluor 488, Rhodamine Phalloidin); Photos were taken using a CFI Plan Apo lambda 60x objective lens and DS-Qi2 camera, provided by Josh Rappoport from the Nikon Imaging Center at Northwestern University; The specimens were provided by S. Kemal, B. Wang, and R. Vassar from Northwestern University.

|NAMC (Nikon Advanced Modulation Contrast)

This is a high contrast imaging technology compatible with plastic sheets. It is suitable for unstained transparent samples, such as oocytes. NAMC provides simulated 3D images through projection effects. Users can easily adjust the contrast direction for each specimen.

NAMC provides simulated 3D images through projection effects

Nikon Advanced Modulation Contrast (NAMC) Image:
Mouse embryos captured using CFI S Plan Fluor ELWD NAMC 20x objective lens

|Automatic calibration ring (Ti2-E)

The thickness of the specimen, the thickness of the cover glass, the distribution of refractive index of the specimen, and changes in temperature may all lead to spherical aberration and image distortion. The highest quality objective lenses are often equipped with adjustment rings to compensate for these changes. The accurate adjustment of the correction loop is the key to obtaining high-resolution and high contrast images. This new automatic correction ring uses harmonic drive and automatic correction algorithm to help users easily adjust to the optimal position every time, thereby maximizing the performance of the objective lens.

Harmonic drive mechanism for precise control of calibration loop regulation

Ultra high resolution images (DNA PAINT):
CV-1 cells expressing alpha microtubule protein (green) and TOMM-20 (magenta) were imaged using a CFI Apo TIRF 100x Oil lens.

|Epifluorescence

The λ series objective lens adopts Nikon's patented Nano Crystal Coat technology, making it an ideal choice for high demand, weak signal, multi-channel fluorescence imaging. Because these applications require the system to maintain high transmission efficiency and aberration calibration over a wide wavelength range. The new fluorescent filter block has a higher fluorescence transmittance and features technologies such as noise terminator to eliminate stray light. Combined with such fluorescent filter blocks, the λ series objective lens has demonstrated its ability in weak fluorescence observation, including single-molecule imaging and applications based on cold light.

Harmonic drive mechanism for precise control of calibration loop regulation

Cold light image:
Expression of BRET based calcium indicator protein and nano calcium cage in Hela cells.
The specimen was provided by Dr. Takeharu Nagai from the Institute of Science and Industry, Osaka University, Japan

|Perfect focus

Even the slightest changes in temperature and the slightest vibrations in the imaging environment can greatly affect focal plane stability. Ti2 adopts both static and dynamic measures to eliminate focal plane shift, allowing for the realistic presentation of macroscopic and microscopic phenomena in long-term experiments.

|Mechanical redesign to achieve ultra-high stability (Ti2-E)

To improve focusing stability, the automatic focusing structure of the electric Z-axis and Perfect Focus System (PFS) has been thoroughly redesigned. The new Z-axis focusing structure has a smaller size and is located adjacent to the objective lens turntable to minimize vibration to the greatest extent possible. Even in extended (dual layer optical path) configurations, it is located adjacent to the objective disc, ensuring excellent stability in all applications.

Even in extended configurations, the Z-axis focusing structure with high stability is located adjacent to the objective turntable

The detector part of the Perfect Focus System (PFS) has been separated from the objective disc to reduce the mechanical load on the objective disc. This new design can also minimize heat transfer to the greatest extent possible, helping to create a more stable imaging environment. Therefore, the power consumption of the electric Z-axis motor has also been reduced. These mechanical redesigns endow the imaging platform with extremely high stability, making it highly suitable for single-molecule imaging and ultra-high resolution applications.

|The new generation autofocus structure using PFS: perfect (Ti2-E)

The latest generation of Perfect Focus System (PFS) is capable of automatically correcting focus drift caused by temperature changes and mechanical vibrations (which are often introduced when adding reagents and multi-point imaging to specimens).

PFS real-time detects and tracks the position of the reference surface (such as the cover glass surface when using immersion lenses) to maintain the focal plane. The unique optical compensation technology allows users to maintain the focal plane at any relative position on the reference plane. Users can directly focus on the desired plane and then enable PFS. PFS works automatically through a built-in linear encoder and high-speed feedback mechanism, while maintaining the focal plane, providing highly reliable images even in long and complex imaging tasks.

PFS is compatible with various applications, from routine experiments on plastic culture dishes to single-molecule imaging and multiphoton imaging. It is also compatible with various wavelengths, from ultraviolet to infrared, which means it can be used for multiphoton and optical tweezers applications.

|Auxiliary guide

No longer need to remember complex microscope calibration and operation steps. Ti2 can integrate data from sensors, guide you through these steps, avoid human error, and enable researchers to focus on the data.

|Continuously display microscope status (Ti2-E/A)

A series of built-in sensors detect and transmit the working status information of various components of the microscope. When you use a computer to capture images, all status information will be recorded in metadata, ensuring that you can easily retrieve capture conditions and/or check for setting errors. In addition, the built-in camera allows users to view the back focal plane, making it easy to calibrate the phase difference ring and DIC extinction cross. It also provides a safe laser calibration method for applications such as TIRF.

Built in sensors detect the status of microscope components

The microscope status can be viewed through both the tablet and the status indicator light on the front panel of the microscope. This makes it possible to conduct status checks in the darkroom as well.

LED

|Operation Procedure Guide (Ti2-E/A)

The auxiliary guide function of Ti2 provides interactive step-by-step guidance for microscope operations. This feature can be viewed on a tablet or computer and integrates real-time data from built-in sensors and internal cameras. The auxiliary guide can assist users in setting up experiments and troubleshooting.

|Automatic error detection (Ti2-E/A)

By using Check Mode, users can easily confirm whether all corresponding microscope components of the selected observation method are in place on their tablet or computer. When the selected observation method fails to be implemented, this inspection mode can reduce the time and effort required to troubleshoot. This feature is particularly useful for multi-user environments, as each user may change the microscope settings. Users can also pre program custom inspection programs.

Display components with incorrect settings

|Intuitive operation

The Ti2 has undergone a thorough redesign - from the overall body structure to the selection and layout of every button and switch - bringing the ultimate user experience. These controls can be easily used even in the darkroom (most experiments are conducted in the darkroom). Ti2 provides an intuitive and easy-to-use user interface, ensuring that researchers can focus on data rather than microscope operations and controls.

|Carefully designed layout for microscope control (Ti2-E/A)

All buttons and switch layouts are based on the lighting type they control. The button for controlling transmission observation is located on the left side of the microscope, while the button for controlling fluorescence observation is located on the right side. The buttons used to control regular operations are located on the front panel. This partitioning method is easy to remember and is particularly practical when operating microscopes in a dark room.

❶ Reciprocating switching (Ti2-E)

The microscope design integrates reciprocating switching to control devices such as the fluorescent filter disk and objective disk. These switches simulate the feeling of manually rotating the above device, achieving intuitive control. These reciprocating switches can also integrate other functions to ensure that a single switch can operate multiple related devices. For example, the reciprocating switching of the fluorescent filter block turntable can not only rotate the turntable, but also switch the fluorescent shutter when the user presses the switch. In addition, these switches can be programmed to operate the emission filter disk and external phase difference units.

❷ Programmable function buttons (Ti2-E/A)

The design of shortcut keys can facilitate users to customize functions. Users can choose from over 100 functions, including control of electric devices such as shutters, and even a single output to external devices through I/O ports used for trigger based acquisition. You can also specify mode functions for these buttons, so that you can switch observation modes at any time by saving each electric device.

❸ Focus knob (Ti2-E)

The focus acceleration button and the Perfect Focus System (PFS) enable button are located next to the focus knob. According to different shapes, it is very easy to recognize buttons with different functions through touch. The focusing speed is automatically adjusted according to the currently used objective lens. This allows users to achieve their desired focusing speed under different objectives, making microscope operation very easy.

|Visual control using joystick and tablet (Ti2-E)

The Ti2 control lever can not only control the movement of the stage, but also control most of the electric functions of the microscope, including the activation state of the Perfect Focus System (PFS). It can display XYZ coordinates and the status of microscope components, greatly facilitating remote control by users. Users can also control the electric functions of Ti2 through a tablet connected to the microscope via wireless LAN, achieving a comprehensive visual operation experience of the microscope.