Product Introduction:

GB-T 18979-2003Determination of aflatoxins in food The standards for purification by immunoaffinity chromatography, high-performance liquid chromatography, and fluorescence spectrophotometry specify the purification by immunoaffinity chromatography—Purification by High Performance Liquid Chromatography and Immunoaffinity Chromatography—Conditions and detailed analysis steps for determining aflatoxins in food by fluorescence spectrophotometry

This standard is applicable to the determination of aflatoxins in corn, peanuts and their products (peanut butter, peanut kernels, peanuts), rice, wheat, vegetable oils, soy sauce, vinegar and other foods.
Detection limit of aflatoxin in samples: purification by immunoaffinity chromatography—Purification by High Performance Liquid Chromatography and Immunoaffinity Chromatography—The fluorescence photometry method is1mg/kgImmunoaffinity chromatography purification—The detection limit of aflatoxin in soy sauce samples determined by fluorescence spectrophotometry is2.5mg/kg.
Immunoaffinity chromatography purification high performance liquid chromatography
Method Summary
Sample passed through methanol-Water extraction, the extract is filtered and diluted, and the filtrate is purified by immunoaffinity chromatography containing specific antibodies against aflatoxinB1TheB2TheG1TheG2Specific, aflatoxin crosslinks onto antibodies in the chromatography medium. Water or Tween/PBSRemove impurities from the immunoaffinity column, elute with methanol through the immunoaffinity chromatography column, and determine the content of aflatoxin by derivatization with iodine solution after passing through a high-performance liquid chromatography column with a fluorescence detector.
Instruments and equipment
Routine laboratory instruments, equipment, materials, and the following items.
1 High speed homogenizer:18,000 r/min ~22,000 r/min.
2 Aflatoxin immunoaffinity column.
3 Fiberglass filter paper: diameter11cm, aperture1.5μm.
4 Glass syringe:10 mL，20mL.
5 Glass test tube: diameter12 mm, Long75mmNo fluorescence characteristics.
6 High performance liquid chromatography: equipped with360nmExcitation wavelength and greater than420nmFluorescence detector with emission wavelength.
7 Air pressure pump.
8 Micro injector:100mL.
9 Chromatographic column:C18Column (column length)150 mm, inner diameter4.6mm, packing diameter5mm）.