Non invasive, online real-time monitoring system for biomass in shake flasks
The CGQ system produced by Aquila Biolabs is an online real-time monitoring device for biomass in shake flasks. During measurement, there is no need to remove the shake flask from the shaker or stop the shaker operation.
The CGQ system automatically and non invasively measures biomass concentration through optical measurement technology. Therefore, high-precision microbial growth kinetics curves can be obtained through measurement results.
Product Features
>Non invasive (placed at the bottom of the culture bottle, not in contact with the culture medium)
>Good sustainability, will not affect the growth of microorganisms/cells
>Automatic measurement
>Save operational time and costs
>Real time measurement
>React quickly to deviations
>Large amount of data collection
>Detailed monitoring of the process within the set time
>Parallel reaction monitoring
>Can simultaneously monitor 16 shaking bottles
Applicable shake bottle size
250mL, 300mL, 500mL, 1000mL, 2000mL
application
Cultivation of bacteria, yeast, cells, etc
cover an area
Sensor board: Ø 92-177 mm
Signal collector base: area ≥ 44 x 105 x 109 mm (L x W x H)
compatibility
Suitable for Biostream shaker and standard products produced by manufacturers
The four major components of CGQ system
1. The sensor board is installed under the shaking bottle for non-invasive measurement of biomass. There are 5 sizes to match different specifications of shaking bottles for selection;
2. Each shaking bottle is covered with a light shield to ensure measurement accuracy and sensitivity;
3. The signal collector base can be connected to 8 or 16 shaking bottles to send data to the cgQuant software;
4. The cgQuant software analyzes and visualizes the data from monitoring shaking bottles;
application
CGQ system can be used for various scientific applications
>Protein expression guided by growth curve
>Development/optimization of culture medium
>Strain screening/comparison
>Monitoring limiting factors and bacterial contamination
>Analyze the growth kinetics curve
>Optimize cultivation conditions
>Online monitoring of thermophilic microorganisms
Technical Parameter
shake flask |
|
Number of shaking bottles monitored simultaneously |
|
8-bit basic unit |
Simultaneously monitor 1-8 shaking bottles |
16 bit basic unit |
Simultaneously monitor 1-16 shaking bottles |
Applicable shaking bottle size |
250ml, 300ml, 500ml, 1000m, 2000ml |
Applicable shaking bottle types |
Glass and fully transparent disposable shaking bottles; Shake bottles with and without baffles |
Compatible with shake bottle sealing caps |
Aluminum lid, tin foil, cotton lid, disposable lid |
Liquid volume |
|
Z is suitable |
10-15% of the total shaking bottle volume |
better |
5-25% of the total shaking bottle volume |
acceptable |
2-30% of the total shaking bottle volume |
Scalable |
<2% of the total shaking bottle volume and>30% of the total shaking bottle volume |
table |
|
Fixtures and adhesive pads |
|
Shake bed fixture |
The sensor can be directly installed into the fixture |
Fixtures from other manufacturers |
The sensor can be installed on the shaker through an adapter |
Adhesive pad |
The sensor can be installed on the shaker through an adapter |
temperature range |
10-50℃ |
Humidity range |
0-80% (non condensing) |
Frequency |
|
Equipped with fixtures and amplitude ≤ 2.5cm |
0-300rpm |
Equipped with fixtures and amplitude ≤ 5.0cm |
0-250rpm |
Adhesive pad |
0-200rpm |
Measurement range and accuracy (reference for actual measurement) |
|
Testing Microorganisms |
E. coli, S. cerevisiae, B. subtillis, P. aeruginosa, S. acidocaldarius |
Test culture medium |
LB, TB, YPD, Brock, Different inorganic salts and basic culture media (such as M9) |
Measurement range and accuracy |
OD600 0.2-50 |
computer |
|
Computer requirements |
Windows 7 or later; Dual core @ 2 GHz/core; RAM => 4GB, HDD => 1GB |