BIONOON 2415 is a chlorophyll extractor specially developed for the determination of chlorophyll-a in water quality.

Application of BIONOON 2415 standard method for extracting chlorophyll:

1. Filtration: Add magnesium carbonate suspension before filtration to prevent pigment decomposition. Negative pressure cannot exceed 50kPa.

2. Extraction of chlorophyll: Place the filter membrane in the BIONOON 2415 extractor, add 10ml of 90% acetone solution, and grind at 1500-2000rpm for 1 minute. Crushing phytoplankton and algae cells on the filter membrane. After grinding, soak and extract in a dark place at 4 ℃ for 15 minutes to 24 hours. Finally, centrifuge and clarify: centrifuge 1000g for 20 minutes or filter, and transfer the supernatant to a stoppered glass vial.

3. Measurement method: spectrophotometry, measuring the absorbance at 664nm, 647nm, 630nm, and 750nm using a three color equation to calculate the chlorophyll a, b, and c content. Measurement of corrected content of pheophylline a using monochromatic equation

BIONOON 2415 can simultaneously process 24 × 15 mL centrifuge tubes for rapid cell lysis, cell lysis, and tissue homogenization.

Grinding samples include plant roots, stems, leaves, flowers, fruits, seeds, and certain animal tissues; Specially suitable for extracting nucleic acids, proteins, and other components from plant tissues. In addition, it can also crush yeast, cultured animal cells, bacterial cells, etc. to extract their components.

The up and down vertical oscillation mode makes the sample grinding more uniform and sufficient, with good sample repeatability and no cross contamination between samples, making it a true high-throughput grinding oscillation system. Each sample only takes 1-2 minutes to complete.

For temperature sensitive samples, BIONOON Tech accessories can be selected for low-temperature grinding and storage.

BIONOON 2415 Applicable samples and applications

1. PCR (polymerase chain reaction) rapid multi sample grinding preparation

2. Rapid lysis of tissue homogenate for nucleic acid extraction, RNA/DNA extraction, and protein extraction

3. Cracking of yeast and bacterial cells

4. Pesticide/Insecticide Analysis (Sample Processing of Food Residue Pesticides or Toxic Substances in LC/MS/MS Testing)

5. QuEChERS method (pesticide, antibiotic, drug extraction)

6. Extraction of Medicinal Active Ingredient APIs

7. Food safety (contamination, adulteration, food traceability)

8. Biofuels

Typical application areas: Molecular marker assisted breeding, genomics, systems biology and molecular evolution, transgenic research, and other biochemical research fields for plant tissue nucleic acid extraction.

To separate nucleic acids from intact seeds, the seeds need to be mechanically crushed before extracting and purifying the nucleic acids. The usual mechanical crushing method is slow and prone to cross contamination, making it unsuitable for high-throughput seed crushing.

useBIONOON 2415Grinding the seeds in the microplate can quickly release a large amount of nucleic acid from the seed cells; Separate and purify the nucleic acid from the homogenate solution. For example, soaking soybeans overnight in water and homogenizing them into a slurry within 1 minute is used as a material source for DNA analysis.

Rapid extraction of genome from cultured cellsD N AbyP C RPrepare for analysis

PCR technology has improved the efficiency of nucleic acid detection and quantitative sequencing. But before template amplification, a step including cell collection and nucleic acid dissolution purification is required, which is a slow process. Then use chromatography resin to separate the nucleic acid from the lysate. It requires a lot of time and expensive materials.

BIONOON 2415The technology rapidly breaks down a large number of cultured cells, preparing for PCR analysis of genomic DNA in the later stage.BIONOON 2415High throughput homogenization treatment is performed on a large number of cultured cells in a microplate, followed by purification of nucleic acids through chromatography resin for PCR analysis, greatly improving the efficiency of genome analysis.

YeastYeast's9 6Hole high flux fragmentation

Yeast has become a universal host for gene expression research and protein recombinant expression, a model organism for biological system research, and a powerful tool for biopharmaceuticals. Picbia, Hansenula, and Debaryomyces have all been used by researchers, such as the most common Saccbioatomyces yeast. Yeast mRNA and intracellular proteins are difficult to extract using traditional enzymatic methods. Lysases typically contain ribonucleases and other proteins that not only attack cell walls, but also specific molecules. Moreover, the protoplasts produced by enzymatic hydrolysis require the use of specific reagents for dissolution, resulting in the denaturation and inactivation of many proteins.

The usual pressing or ball milling methods can only crush yeast cells and release their internal solutes under a single sample, and the operational efficiency is too low.BIONOON 2415A deep hole plate for crushing seeds was designed specifically for experiments that require a large number of yeast clones for high-throughput screening and detection. Yeast was crushed in the deep hole plate. Realize high-throughput cell division.

Bacterial cell lysis (halophilic bacteria and bacilli)Bacterial Cells

BIONOON 2415Using collision to lyse bacterial cells. Two corresponding techniques were developed using Halomonas elongate, a Gram negative salt tolerant bacterium, and Gram positive bacteria as research subjects: 1. Bacterial culture, harvesting and washing off excess culture medium, and suspending cells in a saltwater solution in a deep well plate. 2BIONOON 2415By using grinding media, cells can be shaken and broken, and a sufficient amount of nucleic acid can be released within 6-9 minutes for subsequent experiments.

Main features:

A specially designed grinder has been developed for the application of extracting nucleic acids, proteins, and their analytical components from plant samples

High throughput: It can grind 24 * 15ml samples simultaneously and is suitable for grinding samples of various sizes, greatly improving work efficiency

Each sample only takes 1-2 minutes to complete

To ensure the reproducibility, effectiveness, and comparability of the samples, the grinder can set the grinding time, grinding rate, and number of cycles to ensure each time

Consistency of grinding and next grinding conditions, with a maximum time of 99 minutes.

Equipped with two safety locks and a sample compartment cover interlock, it is extremely safe and reliable.

Expert in grinding animal and plant samples at room temperature and low temperature

The start and stop buttons allow the user to start or stop the grinding process at any time.