1、 Main purpose:Used for microbial colony counting analysis, inhibition zone measurement, antibiotic susceptibility potency analysis, sulbactam sensitive β - lactam enzyme assay, lactomycin potency analysis, and microscopic cell counting analysis

1) ★ Microscopic imaging: Olympus CX31 three eye biological microscope (including a 10 megapixel camera), with a minimum resolution of~1um.

2）★Canon EOS DSLR camera with ultra large zoom lens24.2 million pixel true color CMOS chip

3) Fully enclosed dark box to eliminate environmental stray light interference
4) Flexible shadowless upper light source (high brightness tri color LED surround structured light) to create a clear floating dark field colony imaging effect
5) Uniform and shadowless illumination from a lower light source to highlight the morphology of bacterial colonies and achieve intelligent recognition and counting in a foolproof manner
6) Up light source, down light source, dual light source, UV light source, can be freely combined and switched to obtain the best colony imaging

7）★Equipped with 9-point autofocusParallel observation of dynamic and static images, automatic white balance, and automatic color temperature control. The system contains a scanner interface and can also be analyzed through scanner imaging

8) It has functions such as image enhancement, chromaticity adjustment, grayscale negative phase transformation, image brightness, contrast, and saturation adjustment, and the adjustment values are automatically memorized and saved. Equipped with visual navigation, arbitrary zoom in/out, and local observation functions

9) ★ Suitable for culture dishes: 50-140mm (pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet)

1) ★ Automatic recognition and statistics: SmartdownTM colony intelligent recognition technology automatically recognizes large-scale clustered and chain like adhered long rod-shaped bacteria (microscopic morphology of large adhered Escherichia coli, bacterial spores) and various types of colonies on petri dishes (including Staphylococcus aureus count (GB4789.10-2016), Escherichia coli group determination (GB4789.3-2016), etc.). Automatically generate batch processing wizard to achieve one click automatic counting

2) The automatic counting accuracy is ≥ 96.5%, with a maximum monitoring correction of 3.5%, achieving 100% accuracy. Analysis and statistics speed: 150-800 colonies/s

★ Colony adhesion segmentation: Automatically segment long, circular, and other colony targets that are adhered to each other, and users can choose to segment or not segment them. It is also possible to manually segment and merge colony target images

4) ★ Color and shape recognition:Equipped with fast level set extraction technologyIt can identify and count the number of colonies of specific colors and shapes according to 20 categories, and can be corrected by category conversion. 6 target areas can be selected simultaneously for automatic analysis, and the analysis target areas can be freely increased, decreased, or edited

5) Colony morphology analysis: Automatically obtain colony area, equivalent diameter, long axis, short axis, aspect ratio, roundness, perimeter, shape factor, etc. You can filter and query based on morphological indicators.

6) Click the mouse to correct,Untrace removal of grids and textAutomatically remove impurities, effectively support complex microbial statistics, and comply with the parameter automatic conversion characteristics of GB4789.2-2016.

 1) One click fully automatic determination of all antibacterial zones in the petri dish, which can automatically determine the antibacterial zones that are locally adhered. In the presence of local text interference, the antibacterial zone area, diameter, and other results can be automatically obtained. Measurement and analysis take 1-2 seconds, and can save or view various types of analysis data uniformly

2）★The petri dish can be placed in any directionIt has the functions of automatic selection of antibacterial zone samples, faithful visualization comparison, and editing. The reading resolution error of the antibacterial zone measurement system is ≤ 0.001mm, and the repeated measurement error is ≤ 0.3%; The measurement error of potency is ≤ 0.3%; Repeated measurement error of potency ≤ 0.3%; Measurement difference between stations ≤ 0.2%

 3) ★ Complies with the Chinese Pharmacopoeia, USP United States Pharmacopeia, and EP European Pharmacopoeia, and can complete the latest National Pharmacopoeia (2020 edition) first, second, and third dose and combined calculations, as well as antibiotic potency analysis for all strains.Combination optimization analysis characteristics with potency.

★ According to the food additive Streptococcus lactiae QB 2394-2007 standard, all inhibition zones on the petri dish can be automatically measured and the potency can be automatically analyzed

★ Built in with the latest 25th edition (2015) of the American CLSI "Antimicrobial Susceptibility Test Execution Standard", providing a data input and modification platform for easy updates, and providing a quick tool for paper based drug susceptibility analysis

6) ★ The system administrator can assign usage permissions to more than 10 operators, and the operation records can be traced back to meet GMP requirements

According to the "Method for the Detection of β - Lactamases Sensitive to Sulbactam in Milk and Dairy Products - Cup Plate Method" issued by the Ministry of Health of China, measure the content of β - lactamases sensitive to sulbactam in milk and dairy products. The diameter of the inhibition zone generated by tubes (A), (B), and (D) in the pure water control can be used to automatically determine whether the analysis results of the six petri dishes are valid. A positive or negative result output report can be automatically generated based on the diameter of the inhibition zone generated by tubes (A) and (B) in the sample.

2) It has an automatic warning function for invalid reports, which can automatically remove impurities or defects based on differences in size, making the report more comprehensive and reliable. It can generate reports that comply with GLP/GMP requirements, adhere to the integrity of raw data and records, and provide comprehensive data support for laboratory certification. Historical data can be accessed freely, facilitating digital file management in the laboratory.

1) Image acquisition, observation, and comparison: Capture active targets at regular intervals and save images in formats such as Jpeg, BMP, PNG, TIFF, etc. It can continuously record the sequence of activity target images and store them in AVI format. Image scaling, rotation, mirroring, local observation, mesh mask, etc; Compare and contrast the processed image with the original image

★ Real time preview saturation warning: The saturation warning feature during preview allows you to avoid capturing overexposed images

3）★Real time puzzle and ultra large depth of field expansionAutomatically concatenate multiple views to obtain a complete image. Automatically generate multi focus 3D high-definition images from images captured on multiple focal planes, revealing image details

4) Selection of interest target area: Replace, merge, subtract, intersect any interest target area with circles, rectangles, polygons, and can be edited, deleted, or unselected

5) Color processing and effect enhancement: automatic correction of light field non-uniformity (★ automatic background correction), color adjustment, image color separation and filtering, etc. Image brightness, contrast, saturation, RGB three color components, grayscale coefficient, erosion, dilation, hole filling, inverse adjustment, various histogram correction, smoothing, sharpening, denoising, edge detection, etc.

6) ★ Automatic segmentation of long or circular adhesive targets, automatic classification, counting, and measurement (obtaining morphological parameters such as area, perimeter, diameter, main axis, and secondary axis)

★ Automatic learning classification features based on color and shape, interactive conversion of classification categories, and classification enhancement function based on expert guidance for secondary self-learning

 8) ★ Analyze the target sorting output (by parameters such as area, size, aspect ratio, etc.), and automatically provide a histogram of cell size distribution. It also includes: adding or deleting by setting the area or clicking with the mouse, erasing a single eraser, and erasing the area; Query or filter particles based on specific criteria such as area and perimeter; List of particles for heavy duty, view and output measurement parameters for individual particles

9) Image measurement: measuring line segments, multiple line segments, spline curves, arcs, circles, ellipses, rectangles, polygons, irregular shapes, lengths, areas, perimeters, angles, etc., with caliper tools. Online calibration of measurement units, magnetic lasso, vectorization of all shapes, editable, and arbitrary annotation on images (boundaries, numbers, symbols, fillers, etc.)

1) The analysis of images and data results can be saved and automatically generate Excel or PDF document reports. Various types of analysis data and image evidence can be saved or viewed uniformly, and a total report can be generated. Automatic size calibration and manual calibration. It has functions such as automatic selection of antibacterial zone samples, visual comparison, interactive precise measurement, and editing. The latest Windows operating interface is easy to use, and the software can be upgraded online independently.

1) One optical imaging host with a Canon EOS DSLR camera and a large zoom lens (switchable between floating dark field/backlight, etc.)

 2) One Olympus CX31 three eye biological microscope (including a 10 megapixel color microscope camera)

3) Wan Shen fully automatic colony counting analysis, inhibition zone measurement (antibiotic sensitivity potency analysis), β - lactase detection, microscopic counting analysis software and lock 1 set

4) One branded computer (Core i5 CPU/8GB memory/hard drive 500GB/23 "color display/wireless network card, Windows operating system environment)

Note: The amount marked with ★ in this technical proposal must be responded to, otherwise it is a significant deviation