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Agilent Bio SEC-3 liquid chromatography column
The Agilent Bio SEC-3 liquid chromatography column represents a breakthrough in volume exclusion chromatography (SEC) technology. This chromatographic
Product details
Agilent Bio SEC-3 liquid chromatography column Features:
Agilent Bio SEC-3 chromatographic column
Agilent Bio SEC-3 HPLC ColumnRepresents a breakthrough in volume exclusion chromatography (SEC) technology. This chromatographic column is filled with spherical, narrow particle size distribution 3 μ m silica gel packing with hydrophilic coating. This thin layer of polymer is chemically bonded to the mechanically stable surface of pure silica gel under strictly controlled conditions, ensuring the achievement of high column efficiency volume exclusion fillers. The Agilent Bio SEC-3 HPLC column is available in pore sizes of 100 Å, 150 Å, and 300 Å, making it suitable for volume exclusion separation of most peptides and proteins.
Agilent Bio SEC-3 liquid chromatography column feature
- The sample loading capacity, stability, and reproducibility of biomolecule separation based on molecular weight size
- Narrower peak shape, higher separation, and better protein recovery
- Faster separation speed than larger particle SEC columns
- Compatible with most aqueous buffer solutions
- Has good stability under high and low salt conditions
performance metrics
| aperture | particle size | Molecular weight range | pH range | Max pressure | flow velocity |
| 100 | 3 µm | 100-100000 | 2-8.5 | 240 bar, 3500 psi | 0.1-1.25 mL/min (7.8 mm inner diameter) |
| 0.1-0.4 mL/min (4.6 mm inner diameter) | |||||
| 150 | 3 µm | 500-150000 | 2-8.5 | 240 bar, 3500 psi | 0.1-1.25 mL/min (7.8 mm inner diameter) |
| 0.1-0.4 mL/min (4.6 mm inner diameter) | |||||
| 300O | 3 µm | 5000-1250000 | 2-8.5 | 240 bar, 3500 psi | 0.1-1.25 mL/min (7.8 mm inner diameter) |
| 0.1-0.4 mL/min (4.6 mm inner diameter) |
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